Optical tools

” Fluorescence Fluctuation Microscopy “

Cells constantly probe the physical properties of their microenvironment through mechanotransduction processes involving continuous and bidirectional transduction of cytoskeletal forces in dynamic reorganisations of integrin-mediated adhesive structures.

Here, our goal is to measure the transduction of mechanical signals into biochemical ones by coupling simultaneously time resolved force measurements (tTFM) and integrins mobility, using both FRAP and multi-FCS experiments

Multi-FCS on actin/ An example of an actin-eGFP cell where a serie of 7 laser spots have been superimposed using a spatial-light modulator. Among those spots, two autocorrelation functions are displayed.

For further details see Antoine Delon webpage


2 thoughts on “Optical tools

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